Updated date:

The 16 DPO Project


About This Page

I recently shared a study on hCG levels at 16dpo (days past ovulation) and outcome on pregnancy. I've received numerous messages about this study. As a result, I'd like to start our own informal survey.

On this page, we will be collecting hCG levels at 16dpo as well as the outcome. As I receive numbers and outcomes, I will update our results.

Every level and outcome posted will help other women, please, join us with your numbers. Also, if you know other women who could contribute, we would greatly appreciate their helps as well.

There's Nothing Love Can't Face Poster

I am not a medical professional. The information I share is meant to supplement the information given you by your doctor. If you feel your doctor is not doing enough for you or not willing to listen to your concerns, I strongly encourage you to take what you've learned here and get a second opinion.


Our Project

please, read this first

Although the original study monitored women having IVF for accuracy, I'd like to open up our unofficial study to all. I've found that with ovulation predictor kits (OPKs) and fertility monitoring, many women have a pretty good idea of when conception occurred.

If you have had your levels checked at 16DPO and have either seen the heartbeat or miscarried, please feel free to leave that information. Simply find your numbers here and leave a comment in the appropriate section.

The reason we ask for results if you've seen the heartbeat or had a miscarriage is because women want to know how your pregnancy turned out. Although women can still miscarry after a heartbeat is seen, the percentage of miscarriages does drop dramatically so we will use the heartbeat as our benchmark of successful pregnancy unless a woman states that she miscarried afterwards.


What is dpo?

This page is examining hCG levels 16 days past ovulation (dpo). This is very early in the pregnancy. To give you an idea, a home pregnancy test (hpt) generally shows a positive result 10 to 14 days past ovulation. For many women, this would mean they were approximately 4 weeks 2 days pregnant. Of course, many women have varying cycles so your physician may date your pregnancy based on the first day of your last menstrual period (lmp) which may not be quite accurate. If you have a good idea of when you ovulated, your dating is more likely accurate.

Keep in Mind

Normal hCG range

at 16dpo is 33-223


Our Results

these results will be updated after we receive numbers

Successful pregnancies (heartbeat found):

25 - 50 hCG at 16dpo -- 45 (IVF), 47 (IVF)

50 - 100 hCG at 16dpo -- 89

100 - 199 hCG at 16dpo -- 180, 191, 135, 191, 170, 135

200 - 299 hCG at 16dpo -- 200

Over 300 hCG at 16dpo -- 390, 444, 1173, 790 (loss of one twin), 876


Unsuccessful pregnancies ending in miscarriage

25 - 50 hCG at 16dpo -- 39

50 - 100 hCG at 16dpo -- 121, 103

100 - 199 hCG at 16dpo -- 152 (IVF), 121, 196, 168

200 - 299 hCG at 16dpo -- 270

Over 300 hCG at 16dpo --

More levels can be found in the comments sections below.


If your levels at 16dpo fall within these numbers, please post your actual level as well as the outcome of the pregnancy. Posts will be deleted after chart is updated. IMPORTANT: Numbers can only be added to the chart if we know the outcome of the pregnancy. Thank you

If your levels at 16dpo fall within these numbers, please post your actual level as well as the outcome of the pregnancy. Posts will be deleted after chart is updated.

If your levels at 16dpo fall within these numbers, please post your actual level as well as the outcome of the pregnancy. Posts will be deleted after chart is updated.

If your levels at 16dpo fall within these numbers, please post your actual level as well as the outcome of the pregnancy. Posts will be deleted after chart is updated.

If your levels at 16dpo are 300 or above, please post your actual level as well as the outcome of the pregnancy. Posts will be deleted after chart is updated.

If you would like to include your numbers but had your levels checked on another day, feel free to leave the day (dpo) you believe you had your test, the actual level and the outcome.

The UK is the first to acknowledge that misdiagnosed miscarriages are indeed a problem. The Royal College of Obstetricians and Gynaecologists has revised its guidelines. If your gestational sac is more than 25mm and/or the CRL is 7mm or more, you should wait a week to verify (if there are no complications). If the measurements are less, you are too early to diagnose. For more information (and something to take to your doctor), please, see my new page:

New Blighted Ovum Guidelines! You ARE Being Diagnosed Too Soon!

Need to contact the author? You may e-mail Cari_Kay at misdiagnosedmiscarriage@gmail.com

300 or over hCG at 16dpo

Kay (author) on March 24, 2014:

@Johanna134: I am so sorry :(

Johanna134 on March 24, 2014:

@Cari Kay 11: I held out for two additional weeks hoping for a change, but the gestational sac did not grow at all. No heartbeat, fetal pole or yolk sack was ever seen. Believe me, I wish things had turned out differently for me. But I did lose this pregnancy, even though my betas were nice and high.

Kay (author) on March 14, 2014:

@Johanna134: Hi Johanna. A blighted ovum should not be even suggested before nine weeks. I couldn't even see my baby at eight weeks. Please, get a second opinion. Everything else looks good. Of course, anything can happen but, wow, way too early to know.

Johanna134 on March 13, 2014:

My Betas at 15dpo were 441 and at 17dpo they were 1226. I was just informed that I have a blighted ovum and should expect to miscarry. I'm sorry that this project did not prove true for me. I held out so much hope and promise as my betas kept doubling all the way up to my first ultrasound at 6w2d when we found out it was over.

anonymous on September 05, 2013:

My daughter who's three: 16 dpo - 643. (I posted this same comment on the wrong thread above. Please disregard. Sorry.)

anonymous on July 20, 2013:

1333, lost one twin early(7w), had one healthy boy

anonymous on May 27, 2013:

844 16 dpo. At my 6w2d appt we confirmed twins and had two heartbeats.

anonymous on March 29, 2013:

16dpo morning had 511.90 Waiting for the first visit on the 8th of April I will be 6 weeks... The doctor said over the phone that we might expecting twins....!

anonymous on March 22, 2013:

At 16 dpo, mine was 392. I saw a heartbeat at 6 weeks, 3 days and it was a singleton.

anonymous on November 02, 2012:

200 16dpo

anonymous on October 12, 2012:

Mine was 546 at 16dpo. I saw the heartbeat at 7 weeks and we are not having twins.

anonymous on September 17, 2012:

HCG at 16 dpo: 720Saw the heartbeat but miscarried; baby never got bigger than 6w0d. Also had an enlarged yolk sac.

jenefantony on August 30, 2012:

HCG 16dpo 597

anonymous on July 11, 2012:

16 dpo = 350

anonymous on June 18, 2012:

16 DPO HCG was 498. It ended with a healthy baby girl in our arms.

anonymous on June 06, 2012:

14 DPO = 228, 16 DPO = 681, Strong heartbeat and measuring perfectly at u/s at 5w6d and 7w6d. Natural miscarriage at 8w2d. No known reason. :(

anonymous on May 21, 2012:

16dpo=484, 18dpo=954, 7w4d saw h/b of 130bpm, missed m/c 10w1d (baby measuring only about 8 weeks). 16dpo theory was definitely wrong for me. :(

anonymous on May 03, 2012:

14dpo 14716dpo 441previous 2 early miscarriages(5 weeks and 6 weeks) but no previous beta data. Current at 5w+1 and getting an u/s tomorrow to check all is in the right place.

anonymous on April 21, 2012:

14dpo (IVF) 275 hcg16 dpo 355

anonymous on April 16, 2012:

14 dpo 15416 dpo 335Ultrasound in 12 days

anonymous on March 19, 2012:

@anonymous: 29 dpo: saw heartbeat! Am 11 weeks now and doing well. Waiting for my next ultrasound in a week.

anonymous on March 17, 2012:

16dpo= 316

anonymous on March 09, 2012:

16dpo hcg level 428

anonymous on February 07, 2012:

@anonymous: Update: Update:12 dpo: 10014 dpo: 33516 dpo: 66019 dpo: 1660Going tomorrow for an ultrasound to rule out an ectopic, but numbers look good so far!

anonymous on February 06, 2012:

16 dpo in dec 2011 was 59 - m/c 4 days later...16 dpo (today) feb 2012 is 910!!! hoping and praying

anonymous on February 05, 2012:


anonymous on February 02, 2012:

@anonymous: Update...32 DPO HCG was 79,89033 DPO ultrasound showed 7mm embryo with a heartbeat of 120 bpm

anonymous on January 26, 2012:

16 DPO HCG was 45618 DPO HCG was 106925 DPO HCG was 1901826 DPO ultrasound showed gestational sac and 0.2 mm "lump"....hoping this one sticks. My levels are much higher than my other two pregnancies that ended in miscarriages.

anonymous on January 18, 2012:

16DPO - 40118DPO - 81722DPO - 3310Heart beat never seen.

anonymous on January 05, 2012:

390 at 16dpo and 1343 at 19dpo. Saw a healthy heartbeat at 7w.

anonymous on December 08, 2011:

This is my fifth pregnancy but I only have one daughter - hoping this is a sticky one.14dpo - 35816dpo - 828Good luck girls xx

anonymous on December 08, 2011:

This is my fifth pregnancy but I only have one daughter - hoping this is a sticky one.14dpo - 35816dpo - 828Good luck girls xx

anonymous on December 07, 2011:

This is my first pregnancy and first iui cycle.150 on 14 dpo349 on 16 dpo (today)Praying for a healthy pregnancy.

anonymous on November 22, 2011:

507 day 15.3702 day 2013615 day 2341597 day 27HB seen on scan day 34

anonymous on November 02, 2011:

Last week I got 3 positive pregnancy tests so on Monday I went to have my hcg level tested and at 16 dpo my level was 2,972. I go tonight to get it tested again and then I'll update.

anonymous on October 25, 2011:

1525 at 16 dpo (according ultrasound dates) Heart beat seen at 5 weeks 4 days. Yes this seems high!

anonymous on September 17, 2011:

302 at 16dpo and healthy baby girl born at 39 weeks. This had followed an ovarian ectopic which was 82 at 16dpo.

anonymous on August 26, 2011:

In my fourth pregnancy, my hCG at 16dpo was an off-the-charts 1,327. All our previous pg's had ended in miscarriage so I had high hopes... but even that high hCG pg ended at 9 weeks due to Turner Syndrome.

anonymous on August 05, 2011:

16 DPO 375HB 7weeks

anonymous on July 31, 2011:

1420 at 16dpo and healthy baby girl born at 40w3d.

anonymous on June 20, 2011:

1475 at 16 dpo...waiting for u/s at 8 weeks.

anonymous on June 16, 2011:

420 at 16dpo, saw heartbeat of 142 at 7weeks 2 days

anonymous on May 25, 2011:

16 dpo = 542, 18 dpo = 1387heartbeat at 7w2d = 139

anonymous on May 25, 2011:

Had IVF day 3 transfer of 2 embryos. 13dp3dt (16dpo) initial beta hcg = 1201 !!

anonymous on May 20, 2011:

15 dpo 20617 dpo 5666w1d hb 116healthy baby born

anonymous on May 09, 2011:

@anonymous: Heartbeat found at 6 weeks 6 days on abdominal ultrasound. 126 BPM.

anonymous on April 29, 2011:

750 @ 16DPO

anonymous on April 28, 2011:

@anonymous: I had my last beta done yesterday and it was at 9080 on 25 DPO! So far so good! u/s next week to find the heartbeat.

anonymous on April 27, 2011:

12dpo 8814dpo over40021dpo 888024dpo 17300

anonymous on April 19, 2011:

My HCG at 16 DPO was 439.4 (up from a 12 DPO beta of 60). I will have a repeat draw at 24 DPO to make sure the numbers have gone up again and will have an u/s to check for a h/b on 5/6/11. So far so good.

anonymous on April 05, 2011:

14dpo 15016dpo 330Heartbeat at 6weeks & 4 daysCurrently 35 weeks 1 day

anonymous on March 07, 2011:

16 dpo 672.918 dpo 1764

anonymous on August 12, 2010:

15dpo 307Second pregnancyFirst pregnancy was 82 at 16dpo and was ovarian ectopic

anonymous on August 03, 2010:

731 - Pregnancy still going strong at 11.5 weeks.

anonymous on July 14, 2010:

441 at 16dpo blighted ovum

anonymous on June 20, 2010:

16 dpo 476healthy baby girl born 2nd April 2009

anonymous on June 16, 2010:

15dpo 20617dpo 566hb visible at 6w1dcurrently 23 weeks pregnant

anonymous on June 15, 2010:

14 dpo 9116 dpo 300Heartbeat not visible at 5wk6days.Heartbeat seen at 7wks2days.

anonymous on June 11, 2010:

Baby #1:12dpo: 5916dpo: 48425dpo: 12916One healthy baby girl and I'm 26wks today. Perfect on every scan we've had.Heartbeat seen at 6+4 but could have seen earlier.Heartbeat heard at home at 9+6.but with m/c back in Oct 0814dpo - 1118dpo - 8

anonymous on June 10, 2010:

16dpo - 62318dpo - 1477Heartbeat on ultrasound at 6w5d and 8w4dFirst trimester screening at 11w5d - heartbeat seen again & results are "low-risk"Heard heartbeat on doppler at 12w5dAge = 39

anonymous on June 10, 2010:

14dpo 18616dpo 42319dpo 2266Ultrasound at 7weeks showed a strong heartbeat!

anonymous on August 11, 2009:

14DPO- 15216DPO-394

anonymous on July 11, 2009:

14 DPO 12816 DPO 343Outcome unknown

anonymous on May 25, 2009:

Baby #6 for us...At 12dpo~81, 14dpo~288, 16dpo~665...will have a scan in 2weeks :)

anonymous on May 24, 2009:

I am 376 at 16 DPO. This is my sixth pregnancy. This time, used progesterone supplementation after ovulation..........HCG summary:#1 - going great, until no heartbeat at 12 weeks, fetal death 10.4 weeks.#2 chemical HCG unknown (had to be about 25, very faint positive)#3 chemical HCG unknown (had to be about 25, very faint positive)#4 HCG 48 at 17 DPO (slow rise, to 76, then to 100, and empty sac at 6 weeks)#5 HCG 9 at 16 DPO (went down from 17 on 14 DPO)

anonymous on April 05, 2009:

I moved my scan to two weeks later - so @ 12 weeks.Saw dancing baby!Reminder, 16dpo - 1173

anonymous on March 21, 2009:

14DPO-16616DPO-39019DPO-131135DPO-45103Had a scan at 7weeks Saw baby and HB I am currently 18w4d.

anonymous on March 19, 2009:

16 dpo beta was 444.... at 18dpo it was 957. I saw the baby yesterday and 7wks 4 days and we have a heart beat!

anonymous on March 05, 2009:

I am 16dpo today and my hcg was 867 =)

anonymous on March 05, 2009:

I was at 484 at 16 dpo then 2169 at 19 dpo

anonymous on March 03, 2009:

My level at 16dpo was 1173, and at 18dpo is was 3234.I just had my first scan at eight weeks. We saw one baby with a strong heartbeat measuring right on target for my LMP though I know I ovulated late.I will have another scan in two weeks time and will update then.

anonymous on March 02, 2009:

11 Days Past Ovulation - 1616 Days Past Ovulation - 322Will test again in two days.

anonymous on February 28, 2009:

After 2 miscarriages, this pregnancy at approx 13dpo - 203 and 17dpo- 775. Still waiting on scan. Hope all is well this time!

anonymous on February 26, 2009:

At 16DPO-641. Have not had scan yet.

anonymous on February 23, 2009:

This is my second pregnany. At 14 dpo - 2604, 16 dpo - 7602,.I have an appointment for an ultrasound on March 4th, but so far all seems to be fine.

anonymous on February 22, 2009:

With my son my levels were as follows: 13DPO - 114, 15DPO - 361, 17DPO - 779. obviously was a successful pregnancyWith this pregnancy my levels are - 13DPO - 83, 15DPO - 274. Still waiting on scan to check everything is ok.