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The Enzyme Catalase Activity at Different Conditions Lab Report

Enzyme Catalase

Enzyme Catalase

This is a lab report I wrote for Biology 1 (Molecular and Cells) class at CCBC Essex in Spring 2009. This report is my work, and cannot be used without my permission. If you have any question(s) about this report or find any errors, please don't hesitate to leave me a comment.

** Anyone can use this report as a reference of his/her lab report as long as credit is given to me. But please remember that this lab report isn't perfect nor the best reference for the particular experiment.


Living organisms produce enzymes to speed up their chemical reactions (Hershey, 2009). The activity of these enzymes can be either fast, slow, or stopped according to the factors influencing them. This experiment tested the effect of different surface areas, temperatures, and pHs on the reaction rate of the enzyme Catalase, as well as the reusability of the enzyme.

Following are the hypothesis:

  1. As the surface area gets larger, the reaction rate will increase.
  2. The reaction rate will be the fastest when the temperature is at 37°C, and very high temperature (boiling) will denature the enzymes and therefore no reaction will occur.
  3. The optimum pH will be 7, and very high pH (pH 10) will denature the enzymes.
  4. The enzymes are reusable as long as they are not denatured.

Materials and Methods

 Materials, experimental setup, and procedures can be found on pages 73 to 78 in the Biology 110 Lab Manual.


Part 1 - Decomposition of Hydrogen Peroxide with the Enzyme Catalase

The enzyme Catalases in two banana wedges with different surface areas were used to test the effect of the surface area on the enzyme activity. One banana wedge was mashed to increase the surface area. O2 gas was collected using the experimental setup described in the materials and methods section. Results were recorded on Table 1 and graphed on Graph 1.

Part 2 - Effect of Temperature on Enzyme Function

O2 gas was collected using the experimental setup described in the materials and methods section, the enzyme Catalase in chicken liver, and different temperatures. Different temperatures used were 0°C, room temperature, 37°C, and boiled. The results were recorded on Table 2 and graphed on Graph 2.

Part 3 - Is Catalase Reusable?

The reusability of the enzyme Catalase was tested by pouring unused H2O2 over the chicken liver used for previous part. The reusability of H2O2 was tested by putting a fresh cut chicken liver in the liquid left from the room temperature test tube from Part 2. The result was negative, no reaction occurred.

Part 4 - Reaction Rate of Iced Liver Returned to Room Temperature

The data was collected using the iced liver returned to room temperature. The results were recorded on Table 3.

Part 5 - Effect of pH on Enzyme Activity

The effect of pH on enzyme activity was tested using different pHs (3, 7, and 10). The results were recorded on Table 4 and graphed on Graph 3.

Discussion and Conclusion

Part 1 – The experiment supported the hypothesis: As the surface area gets larger, the reaction rate becomes faster. It is so because as the surface area gets larger, bigger area gets in contact with the substrate and react to it, therefore speeds up the reaction. An analogy would be there is a long line at the bank, and 5 tellers handles the business faster than 1 teller. The Graph 1 displays a big difference in the reaction rate between mashed banana and whole banana. The mashed banana, the banana with more surface area, has faster reaction rate than the whole banana, the banana with less surface area.

Part 2 – The experiment refuted the hypothesis of 37oC being the optimal temperature for the enzyme activity (having the fastest reaction rate). This was due to the uncontrolled lab circumstances. Many people were using the incubator and the temperature wasn’t kept stable at 37oC.

Part 3 – The experiment supported the hypothesis: Catalase was reusable and the hydrogen peroxide wasn’t reusable. Results agree with the established biological facts: reacting substances change, therefore aren’t usable, and enzymes don't change, therefore enzymes are reusable (Hershey, 2009).

Scroll to Continue

Part 4 – The experiment supported the hypothesis: Reaction rate of iced liver returned to room temperature is faster than iced liver. This was so because as long as the enzymes don't get denatured, the enzymes react faster as it gets near the optimum temperature (Brooker, 2008).

Part 5 – The experiment supported the hypothesis: Reaction rate is fastest at pH 7. This was true because the optimum pH for the enzyme Catalase is 7. PH 3 wasn’t close to the optimum pH so the reaction rate was slow, and pH10 was too high and it denatured the enzymes.

Literatures Cited

Brooker, Robert J, et al. Biology. New York: McGraw-Hill, 2008.

Hershey, James, et al. Biology 110 Laboratory Textbook. New York: Pearson, 2009.


lucy on July 26, 2014:

thank you for sharing this. i just wanted to kindly ask what grade did you get after? thank you

Comandolf on April 17, 2013:

Hello. I am gathering secondary data to use in my own report, may i use your results?

12345 on October 18, 2012:

Hello sir. May I use your report as a reference for my lab?

W on October 15, 2012:

Thanks, I needed something to compare my data to

Lulu on July 29, 2012:

I have a suggestion, you should write, "Here are the hypotheses", as you mentioned more than one hypothesis, and the word 'are' was used in the sentence, suggesting more than one. :)

writing a lab report on July 16, 2012:

Thanks for sharing this is good info :

gues on June 22, 2012:

you spelt hypotheses wrong

hyunchang (author) on May 14, 2012:


Yes, you can use this hub as you desire. This report was never published, but was written in Spring of 2009.

selina on May 14, 2012:

would you mind if I used your Lab as a guide/source for mine because we're doing a similar project. I will most definitely cite this! Thanks for uploading. P.s. when is the date of publication? I couldn't seem to find it!

effe on May 10, 2012:

Thank you

kelecia 6043 bebe on May 03, 2012:

who invented school?

jhgdfsjf on April 14, 2012:


hyunchang (author) on March 21, 2012:

Yes, you may. But don't blame me if it's not accurate :)

ana on March 21, 2012:

may i use it as a reference to write my report?

2253 on March 05, 2012:

not helpful

LOL jacob on March 03, 2012:

bro r u kidding me lol

jacob on February 22, 2012:

i need to write an introduction for a grade 12 bio lab can i cite you and this lab as a source with your permission??

would be a great help

paramjeet on November 30, 2011:

we were trying to record catalase activity in broad bean at 4 C but not getting any results does that mean that we should inc the temp to 37 c

yonkinjum on November 27, 2011:

thanks for letting us see your tables and graphs

psd on November 08, 2011:

thanks man...u r a life saver..i got so much ideas from this.

KJ on October 31, 2011:

Hello...may i please use this site as a referance to were i got some information from as it helped clear up some thing and helped me beter understand my data?

Rhiannon on October 30, 2011:

May I use a quote from your part 2 conclusion to support the optimal temperature in my enzyme lab for IB bio? I will obviously cite you as a resource in MLA format at the end of my report!

emma on October 30, 2011:

hey can i cite this experiment to compare with my biology lab in order to prove my results ?

aeisha on October 27, 2011:

why is the H2O2 tested its not the enzymes its the subtrate which doesn't need to be reusable.

Beverly on October 15, 2011:

Can i please use the surface area part to support that my hypothesis was proven?

Farrahhh on October 01, 2011:

Wait, I can find the graphs!! aaaahhh!!! where are they?

Farrahhh on October 01, 2011:

wow, AWESOME!!!!!! This is great for my AP Bio class right now!!!! WOOT WOOT! :DDDDDD

J.G. on September 25, 2011:

Can I have permission to use you as a reference for my lab report? I will cite you, of course!

Nimly on September 21, 2011:

Where table 1?

Clozzle on July 04, 2011:

Intro is very short. But thanks

Sam on June 29, 2011:

Thanks- I really needed this for a Bio internal

jamaica land we love..... jamaica nuff problem lol on June 20, 2011:

mi need sum baby waah help mi!!!!!!!!!!! i mean i am in great need of help biology is killing me

fluffy diva from ja on June 20, 2011:

omg i still dnt get it>>>>>>>>>> jah knw bio haaad

passingby on June 20, 2011:

Wanted to say that I liked how you responded to the "idiot" comment. Nice example.

tohru on February 28, 2011:

may i ask?? what is the purpose of performing catalase activity?? and for the step "is catalase reusable??

jomac on December 03, 2010:

Hi, Thanks for this ,it was so helpful. As were some of the suggestions. Well, apart from the tool who called u an idiot!

hyunchang (author) on November 18, 2010:

I am sorry that you wanted to say that after reading my hub. :( If you tell me why I am an idiot, maybe I can stop being one! :)

yi88oou on November 17, 2010:

you r an idiot

hyunchang (author) on November 05, 2010:

Sorry, I only published the text part of the report :(

kyowon331 on November 05, 2010:

hey thank you for your work but this is my first time where do i find the graph

noobcaek on August 19, 2010:

thank! your information was set out so clearly. it's so much better that all those sites that rattle on about nothing. >_

gie on August 09, 2010:

tq,,, it helps me a lot,,,,

nxzkd on July 24, 2010:


Ellem on July 21, 2010:

Thank You! You helped me a lot to understand this topic! =)

... on May 23, 2010:

where is graph 1?

krishna on May 06, 2010:

not tooo complete...

some of the information are still not included

Yasmin on March 24, 2010:

hmmm thank you wouldn't be enough to say..

there are only few great people left in this world

and sure you are one of them :)

thanks that was helpful

lucy :) on March 18, 2010:

thankkkkyou buddie yu jst saved my life :D

hyunchang (author) on December 05, 2009:

Thanks! :)

vadaa on December 04, 2009:

nice analogy..

hyunchang (author) on November 01, 2009:

Thanks! Comments like yours make me want to post more lab reports. :)

chasity on November 01, 2009:

this info is really helpful! it breaks down this lab a lot easier! thanks

chasity on November 01, 2009:

this info is really helpful! it breaks down this lab a lot easier! thanks

hyunchang (author) on October 25, 2009:

Yes you can :)

Anna on October 25, 2009:

can I compare my results to yours in my lab report if cite th source?

hyunchang (author) on October 12, 2009:


I'm very glad my hub helps!

Ashley on October 12, 2009:


I'm doing a similar lab report for my senior year (high school) biology lab and this is exactly what I needed. Thank you for uploading this lab report.

hyunchang (author) on September 30, 2009:

Thank you for the suggestion Diara. I will add that to the report.

Diara on September 30, 2009:

You could have said WHY the surface area affects the rate of reaction

R on September 29, 2009:


hyunchang (author) on September 22, 2009:

No problem :)

some awesome dude on September 22, 2009:

absolutely! thanks man!

hyunchang (author) on September 21, 2009:

Yes, as long as you indicate the source. :)

some awesome dude on September 21, 2009:

Can i use the surface area part as an indirect quote in my lab report?

naz shafeeq on August 09, 2009:

thanks for this, you've been a big help..

Flinki on July 17, 2009:

The intro is quite short...maybe go into enzyme theory?? Talk about active sites, globular proteins, induced fit and lock and key hypotheses n shiz.

But nice work!!

jinki on July 17, 2009:


lumberjack on May 18, 2009:

I'm inspired to upload a lab report!

hyunchang (author) on May 14, 2009:

Thank you! :)

Haunty from Hungary on May 14, 2009:

A fantastic report. No questions. It's all clear cut. lol

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